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KMID : 0904020020180020251
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Journal of Korean Society for Vascular Surgery
2002 Volume.18 No. 2 p.251 ~ p.258
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Measurement of Smooth Muscle Cell Migration on the Micromachined Groove Topology
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유소영/Yoo SY
박찬영/정 석/김동익/이병붕/허세호/한동철/장준근/Park CY/Chung S/Kim DI/Lee BB/Huh SH/Han DC/Chang JK
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Abstract
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PURPOSE: The spreading, orientation, and chemotaxis with the gradient of a chemoattractant of smooth muscle cells (SMCs) were studied on the micro-grooved substrata by the light, fluorescence and scanning electron microscopy. METHOD: Vertical-walled grooves were produced in silicon wafers by the micromachining technique. All grooves were 4~20micrometer deep and 10~80 micrometer wide. SMCs were cultured on each microgroove and examined under stereo-microscope. RESULT: Cell clusters were markedly oriented by all the grooved substrata examined. Time-lapse images acquired from CCD (Charge Coupled Device) showed that the grooves directed the migration of SMCs. There was no prominent difference in the migration speed of SMCs according to the grooves. All the cytoskeletal fibers were reorganized in the same direction with grooves. Especially the alignments of microtubule and intermediate filaments were distinguished in the SMCs on the micro grooves. CONCLUSION: These results could be applied to the analysis of vascular restenosis and the development of artificial blood vessels.
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KEYWORD
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